Multidimensional Separation of Chiral Amino Acid Mixtures in a Multilayered Three-Dimensional Hybrid Microfluidic/Nanofluidic Device

Kim, Bo Young and Yang, J and Gong, MJ and Flashbart, BR and Shannon, MA and Bohn, BW and Sweedler, JV. (2009) Multidimensional Separation of Chiral Amino Acid Mixtures in a Multilayered Three-Dimensional Hybrid Microfluidic/Nanofluidic Device. Analytical Chemistry, 81 (7). p. 2715. ISSN 00032700

Full text not available from this repository. (Request a copy)

Abstract

Microscale total analysis systems (mu TAS) allow high-throughput analyses by integrating multiple processes, parallelization, and automation. Here we combine unit operations of mu TAS to create a device that can perform multidimensional separations using a three-dimensional hybrid microfluidic/nanofluidic device composed of alternating layers of patterned poly(methyl methacrylate) and nanocapillary array membranes constructed from nuclear track-etched polycarbonate. Two consecutive electrophoretic separations are performed, the first being an achiral separation followed by a chiral separation of a selected analyte band. Separation conditions are optimized for a racemic mixture of fluorescein-isothiocynate-labeled amino acids, serine and aspartic acid, chosen because there are endogenous D-forms of these amino acids in animals. The chiral separation is implemented using micellar electrokinetic chromatography using beta-cyclodextrin as the chiral selector and sodium taurocholate as the micelle-forming agent Analyte separation is monitored by dual-beam laser-induced fluorescence detection. After separation in the first electrophoretic channel, the preselected analyte is sampled by the second-stage separation using an automated collection sequence with a zero-crossing algorithm. The controlled fluidic environment inherent to the three-dimensional architecture enables a series of separations in varying fluidic environments and allows sample stacking via different background electrolyte pH conditions. The ability to interface sequential separations, selected analyte capture, and other fluidic manipulations in the third dimension significantly improves the functionality of multilayer microfluidic devices.

Item Type: Article
Uncontrolled Keywords: CAPILLARY-ZONE-ELECTROPHORESIS; CENTRAL-NERVOUS-SYSTEM; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; PERFORMANCE LIQUID-CHROMATOGRAPHY; NANOCAPILLARY ARRAY MEMBRANES; D-ASPARTIC ACID; D-SERINE; NANOFLUIDIC INTERCONNECTS; 2-DIMENSIONAL SEPARATIONS; SAMPLE STACKING
InterNano Taxonomy: Nanoscale Objects and Nanostructured Materials > Nanodevice Structures
Nanoscale Objects and Nanostructured Materials > Nanoparticles > Ferrofluids
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Nanoscale Chemical-Electrical-Mechanical Manufacturing Systems
Nanomanufacturing Research Collection
Depositing User: Danielle Federa
Date Deposited: 17 Jun 2009 14:13
Last Modified: 19 Sep 2011 21:11
URI: http://eprints.internano.org/id/eprint/136

Actions (login required)

View Item View Item