Kyle, J. R. and Kyle, M. D. and Raghavan, R. and Budak, G. and Ozkan, C. S. and Ozkan, M.. (2011) Analysis of light scattering from human breast tissue using a custom dual-optical scanning near-field optical microscope. Journal of Biophotonics, 4 (3). pp. 193-205.Full text not available from this repository.
In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of topographic artifacts. Using this novel dual-optical SNOM, we image desiccated and non-desiccated human breast epithelial tissue. By comparing the simultaneous SNOM images, we isolate the effects of tissue morphology and variations in refractive indices on the forward- and back-scattering of light from the tissue. We find that the reduction in back-scattering from tissue, relative to the glass slide, is caused by dense packing of the scattering sites in the cytoplasm (morphology) in the desiccated tissue and a thin-film of water adhering to the glass slide (refractive index) in the non-desiccated tissue sample. Our work demonstrates the potential of our customized dual-optical SNOM system for label-free tissue diagnostics.
|InterNano Taxonomy:||Nanomanufacturing Characterization Techniques > Diffraction and Scattering|
|Collections:||Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing|
|Depositing User:||Robert Stevens|
|Date Deposited:||03 May 2012 16:35|
|Last Modified:||03 May 2012 16:36|
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