Miranda, O. R. and Li, X. N. and Garcia-Gonzalez, L. and Zhu, Z. J. and Yan, B. and Bunz, U. H. F. and Rotello, V. M.. (2011) Colorimetric Bacteria Sensing Using a Supramolecular Enzyme-Nanoparticle Biosensor. Journal of the American Chemical Society, 133 (25). pp. 9650-9653. ISSN 0002-7863Full text not available from this repository.
Rapid and sensitive detection of pathogens is a key requirement for both environmental and clinical settings. We report here a colorimetric enzyme nanoparticle conjugate system for detection of microbial contamination. In this approach, cationic gold nanoparticles (NPs) featuring quaternary amine headgroups are electrostatically bound to an enzyme [beta-galactosidase (beta-Gap], inhibiting enzyme activity. Analyte bacteria bind to the NP, which releases the beta-Gal and restores its activity, providing an enzyme-amplified colorimetric readout of the binding event. Using this strategy, we have been able to quantify bacteria at concentrations of 1 x 10(2) bacteria/mL in solution and 1 x 10(4) bacteria/mL in a field-friendly test strip format.
|InterNano Taxonomy:||Nanoscale Objects and Nanostructured Materials
Areas of Application > Medical and Pharmaceutical Industries
Nanoscale Objects and Nanostructured Materials > Nanoparticles
|Collections:||Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing|
|Depositing User:||Robert Stevens|
|Date Deposited:||19 Apr 2012 20:42|
|Last Modified:||02 May 2012 18:02|
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