Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

Wang, Sheng and Wong Po Foo, Cheryl and Warrier, Ajithkumar and Poo, Mu-ming and Heilshorn, Sarah C. and Zhang, Xiang. (2009) Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments. Biomedical Microdevices, 11 (5). pp. 1127-1134. ISSN 1387-2176

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Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research.

Item Type: Article
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Scalable and Integrated Nanomanufacturing
Depositing User: Robert Stevens
Date Deposited: 06 Oct 2014 21:00
Last Modified: 06 Oct 2014 21:00

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