Chen, J. H. and Alcaine, S. D. and Jiang, Z. W. and Rotello, V. M. and Nugen, S. R.. (2015) Detection of Escherichia coli in Drinking Water Using T7 Bacteriophage-Conjugated Magnetic Probe. Analytical Chemistry, 87 (17). pp. 8977-8984.
Full text not available from this repository.Abstract
In this study, we demonstrate a bacteriophage (phage)-based magnetic separation scheme for the rapid detection of Escherichia colt (E. coli) in drinking water. T7 phage is a lytic phage with a broad host range specificity for E. coli. Our scheme was as follows: (1) T7 bacteriophage-conjugated magnetic beads were used to capture and separate E. coli BL21 from drinking water; (2) subsequent phage-mediated lysis was used to release endemic beta-galactosidase (beta-gal) from the bound bacterial cells; (3) the release of beta-gal was detected using chlorophenol red-beta-D-galactopyranoside (CRPG), a colorimetric substrate which changes from yellow to red in the presence of beta-gal. Using this strategy, we were able to detect E. coli at a concentration of 1 x 10(4) CFU.mL(-1) within 2.5 h. The specificity of the proposed magnetic probes toward E. coli was demonstrated against a background of competing bacteria. By incorporating a pre-enrichment step in Luria-Bertani (LB) broth supplemented with isopropyl beta-D-thiogalactopyranoside (IPTG), we were able to detect 10 CFU.mL(-1) in drinking water after 6 h of pre-enrichment. The colorimetric change can be determined either by visual observation or with a reader, allowing for a simple, rapid quantification of E. coli in resource-limited settings.
| Item Type: | Article |
|---|---|
| Additional Information: | ISI Document Delivery No.: CQ7HHTimes Cited: 2Cited Reference Count: 48Chen, Juhong Alcaine, Samuel D. Jiang, Ziwen Rotello, Vincent M. Nugen, Sam R.Jiang, Ziwen/H-7538-2012Jiang, Ziwen/0000-0002-6633-7824Center for Hierarchical Manufacturing, a National Science Foundation Nanoscale Science and Engineering Center at the University of Massachusetts CMMI-1025020; USDA 2013-02037Support was provided by the Center for Hierarchical Manufacturing, a National Science Foundation Nanoscale Science and Engineering Center at the University of Massachusetts supported under Award No. CMMI-1025020. We also thank the USDA 2013-02037 for supporting this research.214Amer chemical socWashington1520-6882 |
| Uncontrolled Keywords: | Chemistry |
| Collections: | Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing |
| Depositing User: | Robert Stevens |
| Date Deposited: | 12 Nov 2015 18:38 |
| Last Modified: | 12 Nov 2015 18:38 |
| URI: | http://eprints.internano.org/id/eprint/2322 |
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