Bacteriophage-based nanoprobes for rapid bacteria separation

Chen, J. H. and Duncan, B. and Wang, Z. Y. and Wang, L. S. and Rotello, V. M. and Nugen, S. R.. (2015) Bacteriophage-based nanoprobes for rapid bacteria separation. Nanoscale, 7 (39). pp. 16230-16236.

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The lack of practical methods for bacterial separation remains a hindrance for the low-cost and successful development of rapid detection methods from complex samples. Antibody-tagged magnetic particles are commonly used to pull analytes from a liquid sample. While this method is well-established, improvements in capture efficiencies would result in an increase of the overall detection assay performance. Bacteriophages represent a low-cost and more consistent biorecognition element as compared to antibodies. We have developed nanoscale bacteriophage-tagged magnetic probes, where T7 bacteriophages were bound to magnetic nanoparticles. The nanoprobe allowed the specific recognition and attachment to E. coli cells. The phage magnetic nanprobes were directly compared to antibody-conjugated magnetic nanoprobes. The capture efficiencies of bacteriophages and antibodies on nanoparticles for the separation of E. coli K12 at varying concentrations were determined. The results indicated a similar bacteria capture efficiency between the two nanoprobes.

Item Type: Article
Additional Information: ISI Document Delivery No.: CS8PQTimes Cited: 1Cited Reference Count: 45Chen, Juhong Duncan, Bradley Wang, Ziyuan Wang, Li-Sheng Rotello, Vincent M. Nugen, Sam R.USDA 2013-02037; Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF CMMI-1025020We thank the USDA 2013-02037 for supporting this research financially. Dale A. Callaham is acknowledged for his assistance with the TEM imaging. This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation under NSF Award Number CMMI-1025020. Additional thanks go to Professor Mark Tuominen in the Department of Physics at the University of Massachusetts, Amherst for the use of his SQUID magnetometer. The authors would like to thank Dr Sankar Adhya at the national Cancer Institute for the modified T7 bacteriophage.10Royal soc chemistryCambridge2040-3372
Uncontrolled Keywords: Physics
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing
Depositing User: Robert Stevens
Date Deposited: 12 Nov 2015 18:38
Last Modified: 12 Nov 2015 18:38

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