Multiplexed Screening of Cellular Uptake of Gold Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry

Zhu, Zheng-Jiang and Ghosh, Partha S. and Miranda, Oscar R. and Vachet, Richard W. and Rotello, Vincent M.. (2008) Multiplexed Screening of Cellular Uptake of Gold Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry. Journal of the American Chemical Society, 130 (43). pp. 14139-14143. ISSN 0002-7863

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Abstract

Gold nanoparticles (AuNPs) are highly promising candidates as drug delivery agents into cells of interest. We describe for the first time the multiplexed analysis of nanoparticle uptake by cells using mass spectrometry. We demonstrate that the cellular uptake of functionalized gold nanoparticles with cationic or neutral surface ligands can be readily determined using laser desorption/ionization mass spectrometry of cell lysates. The surface ligands have “mass barcodes” that allow different nanoparticles to be simultaneously identified and quantified at levels as low as 30 pmol. Using this method, we find that subtle changes to AuNP surface functionalities can lead to measurable changes in cellular uptake propensities.

Item Type: Article
Additional Information: Reprinted with permission from "Multiplexed Screening of Cellular Uptake of Gold Nanoparticles Using Laser Desorption/Ionization Mass Spectrometry", V Rotello et al., Journal of the American Chemical Society, 130(43), pg 14139 - 14143. Copyright 2008 American Chemical Society.
InterNano Taxonomy: Nanomanufacturing Characterization Techniques > Other Characterization Techniques > Mass spectrometry
Nanoscale Objects and Nanostructured Materials
Nanomanufacturing Processes > Biological Techniques
Nanoscale Objects and Nanostructured Materials > Nanoparticles
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing
Nanomanufacturing Research Collection
Depositing User: Moureen Kemei
Date Deposited: 17 Feb 2010 22:29
Last Modified: 19 Sep 2011 21:19
URI: http://eprints.internano.org/id/eprint/291

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