Synergistic Effects of Mutations and Nanoparticle Templating in the Self-Assembly of Cowpea Chlorotic Mottle Virus Capsids

Aniagyei, Stella E. and Kennedy, Chelsea J. and Stein, Barry and Willits, Deborah A. and Douglas, Trevor and Young, Mark J. and De, Mrinmoy and Rotello, Vincent M. and Srisathiyanarayanan, D. and Kao, C. Cheng and Dragnea, Bogdan. (2009) Synergistic Effects of Mutations and Nanoparticle Templating in the Self-Assembly of Cowpea Chlorotic Mottle Virus Capsids. Nano Letters, 9 (1). pp. 393-398. ISSN 1530-6984

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Abstract

A study of the in vitro nanoparticle-templated assembly of a mutant of cowpea chlorotic mottle virus lacking most of the N-terminal domain (residues 4−37), NΔ34, is presented. Mutant empty proteins assemble into empty capsids with a much broader distribution of sizes than the wild-type virus. This increased flexibility in the assembly outcomes is known to be detrimental for the assembly process in the presence of molecular polyanions. However, when rigid polyanionic cores are used, such as nanoparticles, the assembly process is restored and virus-like particles form. Moreover, the breadth of the nanoparticle-templated capsid size distribution becomes comparable with the wild-type virus size distribution.

Item Type: Article
Additional Information: Reprinted with permission from "Synergistic Effects of Mutations and Nanoparticle Templating in the Self-Assembly of Cowpea Chlorotic Mottle Virus Capsids", B. Dragnea et al., Nano Letters, 9(1), pp 393–398. Copyright 2009 American Chemical Society.
Uncontrolled Keywords: X-RAY CRYSTALLOGRAPHY; SMALL SPHERICAL VIRUS; CRYOELECTRON MICROSCOPY; CONTROLLED POLYMORPHISM; ESCHERICHIA-COLI; PROTEIN CAGES; COAT PROTEIN; PARTICLES; DELIVERY; SYSTEM
InterNano Taxonomy: Nanomanufacturing Processes > Self Assembly
Nanomanufacturing Processes > Self Assembly > Virus assembly
Nanomanufacturing Processes > Assembly Techniques
Nanomanufacturing Processes > Biological Techniques
Nanomanufacturing Processes > Biological Techniques > Protein assembly
Nanoscale Objects and Nanostructured Materials > Nanoparticles
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing
Nanomanufacturing Research Collection
Depositing User: Moureen Kemei
Date Deposited: 26 Feb 2010 16:01
Last Modified: 19 Sep 2011 20:37
URI: http://eprints.internano.org/id/eprint/311

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