Characterization of Grafting Density and Binding Efficiency of DNA and Proteins on Gold Surfaces

Castelino, Kenneth and Kannan, Balaji and Majumdar, Arun. (2005) Characterization of Grafting Density and Binding Efficiency of DNA and Proteins on Gold Surfaces. Langmuir, 21 (5). pp. 1956-1961. ISSN 0743-7463

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Abstract

The surface grafting density of biomolecules is an important factor for quantitative assays using a wide range of biological sensors. We use a fluorescent measurement technique to characterize the immobilization density of thiolated probe DNA on gold and hybridization efficiency of target DNA as a function of oligonucleotide length and salt concentration. The results indicate the dominance of osmotic and hydration forces in different regimes of salt concentration, which was used to validate previous simulations and to optimize the performance of surface-stress based microcantilever biosensors. The difference in hybridization density between complementary and mismatched target sequences was also measured to understand the response of these sensors in base-pair mismatch detection experiments. Finally, two different techniques for immobilizing proteins on gold were considered and the surface densities obtained in both cases were compared.

Item Type: Article
Additional Information: Reprinted with permission from "Characterization of Grafting Density and Binding Efficiency of DNA and Proteins on Gold Surfaces", Castelino K. et al, Langmuir, 2005, 21 (5), pp 1956–1961. Copyright 2005 American Chemical Society.
InterNano Taxonomy: Nanomanufacturing Processes > Self Assembly > Chemical surface functionalization
Nanomanufacturing Processes > Biological Techniques
Nanomanufacturing Characterization Techniques > Other Characterization Techniques > X-ray photoelectron spectroscopy (XPS)
Collections: Nanomanufacturing Research Collection
Depositing User: Moureen Kemei
Date Deposited: 12 Mar 2010 16:06
Last Modified: 12 Mar 2010 16:06
URI: http://eprints.internano.org/id/eprint/343

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