Super-Resolution Imaging by Random Adsorbed Molecule Probes

Wu, Dongmin and Liu, Zhaowei and Sun, Cheng and Zhang, Xiang. (2008) Super-Resolution Imaging by Random Adsorbed Molecule Probes. Nano Letters, 8 (4). pp. 1159-1162. ISSN 1530-6984

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Abstract

Single molecule localization (SML) is a powerful tool to measure the position and trajectory of molecules in numerous systems, with nanometer accuracy. This technique has been recently utilized to overcome the diffraction limit in optical imaging. So far, super-resolution imaging by SML was demonstrated using photoactivable or photoswitchable fluorophores, as well as diffusive fluorophore probes in solution. All these methods, however, rely on special fluorophore or object properties. In this Letter, we propose and demonstrate a new super-resolution technique attainable for a bio/dielectric structure on a metal substrate. A sub-diffraction-limited image is obtained by randomly adsorbed fluorescent probe molecules on a liquid–solid interface, while the metal substrate, quenching the unwanted fluorescent signal, provides a significantly enhanced imaging contrast. As this approach does not use specific stain techniques, it can be readily applied to general dielectric objects, such as nanopatterned photoresist, inorganic nanowires, subcellular structures, etc.

Item Type: Article
Additional Information: Reprinted with permission from "Super-Resolution Imaging by Random Adsorbed Molecule Probes", Wu D et al., Nano Lett., 2008, 8 (4), pp 1159–1162. Copyright 2008 American Chemical Society
Uncontrolled Keywords: Single molecule localization, high resolution
InterNano Taxonomy: Nanoscale Objects and Nanostructured Materials > Nanoparticles
Collections: Nanomanufacturing Research Collection
Depositing User: Moureen Kemei
Date Deposited: 05 Apr 2010 19:14
Last Modified: 05 Apr 2010 19:14
URI: http://eprints.internano.org/id/eprint/406

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