Synthesis of nanogel-protein conjugates

Matsumoto, N. M. and Gonzalez-Toro, D. C. and Chacko, R. T. and Maynard, H. D. and Thayumanavan, S.. (2013) Synthesis of nanogel-protein conjugates. Polymer Chemistry, 4 (8). pp. 2464-2469.

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Abstract

The covalent conjugation of bovine serum albumin (BSA) to disulfide cross-linked polymeric nanogels is reported. Polymeric nanogel precursors were synthesized via a reversible addition-fragmentation chain transfer (RAFT) random copolymerization of poly(ethylene glycol) methyl ether methacrylate (PEGMA) and pyridyl disulfide methacrylate (PDSMA). Reaction of the p(PEGMA-co-PDSMA) with dithiothreitol resulted in the formation of nanogels. PDSMA serves as both a crosslinking agent and a reactive handle for the surface modification of the nanogels. Lipophilic dye, DiI, was sequestered within the nanogels by performing the crosslinking reaction in the presence of the hydrophobic molecule. Thiol-enriched BSA was conjugated to nanogels loaded with DiI via a thiol-disulfide exchange reaction between the BSA and the surface exposed nanogel pyridyl disulfides. Conjugation was confirmed by fast protein liquid chromatography, dynamic light scattering, and agarose and polyacrylamide gel electrophoresis. We expect that this methodology is generally applicable to the preparation of nanogel-protein therapeutics.

Item Type: Article
Additional Information: ISI Document Delivery No.: 108JRTimes Cited: 1Cited Reference Count: 47Matsumoto, Nicholas M. Gonzalez-Toro, Daniella C. Chacko, Reuben T. Maynard, Heather D. Thayumanavan, S.National Institutes of Health R21 CA 137506-01; Army Research Office 57858CH; National Science Foundation for support through the Center for Hierarchical Manufacturing at UMass Amherst CMMI-0531171; National Science Foundation DGE-0707424; IGERT program through the Institute for Cellular Engineering at UMass AmherstHDM thanks the National Institutes of Health (R21 CA 137506-01) for funding. ST thanks the Army Research Office (57858CH) and the National Science Foundation for support through the Center for Hierarchical Manufacturing at UMass Amherst (CMMI-0531171). NMM thanks the National Science Foundation for a Graduate Research Fellowship (DGE-0707424). DCG thanks for NSF for support by the IGERT program through the Institute for Cellular Engineering at UMass Amherst.Royal soc chemistryCambridge
Uncontrolled Keywords: accumulation
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing
Depositing User: Robert Stevens
Date Deposited: 27 Mar 2014
Last Modified: 27 Mar 2014 20:21
URI: http://eprints.internano.org/id/eprint/2199

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