Protein Array Fabrication with E-jet Printing

Rogers, John A.. (2012) Protein Array Fabrication with E-jet Printing. Center for Nanoscale Chemical-Electrical-Mechanical Manufacturing Systems. (Unpublished)

UIUC NSEC 0749028 John A. Rogers Protein Array Fabrication with E-jet Printing.pdf

Download (222kB) | Preview


We demonstrate the feasibility of fabricate biosensors with direct protein printing without complicated processes at high resolution and area coverage. Printed single protein, streptavidin-fluorescein, binds to biotin labeled with fluorescent DNA without denaturation; therefore a fluorescence microscope can detect the printed droplets as shown in Figure 7.1(a). The standard deviation of the spot size and spacing is 7.26 % and 0.693%, respectively. The e-jet printing is capable to print large area, e.g., one inch size. Half droplets of streptavidin-Cy5 are printed in 15 minutes (Figure 7.1(b)). Four-inch size printing is also available. Moreover, we have developed the multi-syringe head [1]. The head prints multiple inks by rotation. Even complicated patterns of multiple proteins (e.g., peacock shown in Figure 7.1(c)) can be printed. Printed primary antibodies can be detected under a fluorescence microscopy by incubating with fluorescently-labeled secondary antibodies [2]. These capabilities show that the e-jet printer with the multi-syringe head has feasibility to make protein chips to detect multiple proteins at lower cost than the conventional chips.

Item Type: Other
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Nanoscale Chemical-Electrical-Mechanical Manufacturing Systems
Depositing User: Jessica Adamick
Date Deposited: 28 Jan 2013 16:11
Last Modified: 28 Jan 2013 16:11

Actions (login required)

View Item View Item