Rogers, John A.. (2012) Protein Array Fabrication with E-jet Printing. Center for Nanoscale Chemical-Electrical-Mechanical Manufacturing Systems. (Unpublished)
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UIUC NSEC 0749028 John A. Rogers Protein Array Fabrication with E-jet Printing.pdf Download (222kB) | Preview |
Abstract
We demonstrate the feasibility of fabricate biosensors with direct protein printing without complicated processes at high resolution and area coverage. Printed single protein, streptavidin-fluorescein, binds to biotin labeled with fluorescent DNA without denaturation; therefore a fluorescence microscope can detect the printed droplets as shown in Figure 7.1(a). The standard deviation of the spot size and spacing is 7.26 % and 0.693%, respectively. The e-jet printing is capable to print large area, e.g., one inch size. Half droplets of streptavidin-Cy5 are printed in 15 minutes (Figure 7.1(b)). Four-inch size printing is also available. Moreover, we have developed the multi-syringe head [1]. The head prints multiple inks by rotation. Even complicated patterns of multiple proteins (e.g., peacock shown in Figure 7.1(c)) can be printed. Printed primary antibodies can be detected under a fluorescence microscopy by incubating with fluorescently-labeled secondary antibodies [2]. These capabilities show that the e-jet printer with the multi-syringe head has feasibility to make protein chips to detect multiple proteins at lower cost than the conventional chips.
Item Type: | Other |
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Collections: | Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Nanoscale Chemical-Electrical-Mechanical Manufacturing Systems |
Depositing User: | Jessica Adamick |
Date Deposited: | 28 Jan 2013 16:11 |
Last Modified: | 28 Jan 2013 16:11 |
URI: | http://eprints.internano.org/id/eprint/1895 |
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