Lithographic techniques and surface chemistries for the fabrication of PEG-passivated protein microarrays

KANNAN, B and CASTELINO, K and CHEN, F and MAJUMDAR, A. (2006) Lithographic techniques and surface chemistries for the fabrication of PEG-passivated protein microarrays. Biosensors and Bioelectronics, 21 (10). pp. 1960-1967. ISSN 09565663

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This article presents a new technique to fabricate patterns of functional molecules surrounded by a coating of the inert poly(ethylene glycol) (PEG) on glass slides for applications in protein microarray technology. The chief advantages of this technique are that it is based entirely on standard lithography processes, makes use of glass slides employing surface chemistries that are standard in the microarray community, and has the potential to massively scale up the density of microarray spots. It is shown that proteins and antibodies can be made to self-assemble on the functional patterns in a microarray format, with the PEG coating acting as an effective passivating agent to prevent non-specific protein adsorption. Various standard surface chemistries such as aldehyde, epoxy and amine are explored for the functional layer, and it is conclusively demonstrated that only an amine-terminated surface satisfies all the process constraints imposed by the lithography process sequence. The effectiveness of this microarray technology is demonstrated by patterning fluorescent streptavidin and a fluorescent secondary antibody using the well-known and highly specific interaction between biotin and streptavidin.

Item Type: Article
InterNano Taxonomy: Nanomanufacturing Processes > Assembly Techniques > Supermolecular chemical assembly
Nanomanufacturing Processes > Biological Techniques > Protein assembly
Nanomanufacturing Processes > Nanopatterning/Lithography
Collections: Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Scalable and Integrated Nanomanufacturing
Depositing User: Moureen Kemei
Date Deposited: 24 Mar 2010 20:30
Last Modified: 30 Sep 2014 15:39

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