Nugen, S.R.;. Center for Hierarchical Manufacturing. (2012) Microfluidic Sensors for Resource-limited Areas. Center for Hierarchical Manufacturing. (Unpublished)
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Abstract
An ideal point-of-care device would incorporate the simplicity and reliability of a lateral flow assay with a microfluidic device. Our system consists of self-priming microfluidics with sealed conjugate pads of reagent delivery and an absorbent pad for additional fluid draw. Using poly (methyl methacrylate) (PMMA) as a substrate, we have developed a single-step surface modification method which allows strong capillary flow within a sealed microchannel. Conjugate pads within the device held trapped complex consisting of the magnetic beads and nucleic-acid-probe-conjugated horseradish peroxidase (HRP). Magnetic beads were released when sample entered the chamber and hybridized with the complex. The complex was immobilized over a magnet while a luminol co-reactant stream containing H2O2 was merged with the channel. A plate reader was able to quantify the chemiluminescence signal. This new format of biosensor will allow for a smaller and more sensitive biosensor, as well as commercial-scale manufacturing and low materials cost.
| Item Type: | Other |
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| Collections: | Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing |
| Depositing User: | Jessica Adamick |
| Date Deposited: | 05 Oct 2012 18:17 |
| Last Modified: | 05 Oct 2012 18:17 |
| URI: | http://eprints.internano.org/id/eprint/1859 |
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