Polymeric Inverse Micelles as Selective Peptide Extraction Agents for MALDI-MS Analysis

Combariza, Marianny Y. and Savariar, Elamprakash N. and Vutukuri, Dharma Rao and Thayumanavan, S. and Vachet, Richard W.. (2007) Polymeric Inverse Micelles as Selective Peptide Extraction Agents for MALDI-MS Analysis. Analytical Chemistry, 79 (18). pp. 7124-7130. ISSN 0003-2700

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Abstract

Analyses of peptides in complex mixtures are significant challenges in proteomics applications. Here, we report an amphiphilic polymer-based nanoassembly that is capable of selectively extracting peptides, on the basis of their isoelectric points, into an immiscible organic phase from an aqueous solution. The isoelectric point (pI) cutoff in these extractions depends on the pH of the aqueous solution, and thus, sequential fractionation of peptide mixtures based on pI can be accomplished by varying the pH of the aqueous solution. Additionally, we observe an unexpected enhancement in the MALDI-MS signal for extracted peptides ionized in the presence of the polymer, which allows us to obtain reproducible ion signals for some peptides at concentrations as low as 10 pM.

Item Type:	Article
Additional Information:	Reprinted with permission from "Polymeric Inverse Micelles as Selective Peptide Extraction Agents for MALDI-MS Analysis", M.Y. Combariza et al., Analytical Chemistry. Copyright 2007 American Chemical Society.
InterNano Taxonomy:	Nanomanufacturing Characterization Techniques > Other Characterization Techniques > Mass spectrometry Informatics and Standards Nanomanufacturing Processes > Assembly Techniques > Electrostatic driven assembly Nanomanufacturing Processes > Biological Techniques > Protein assembly Nanomanufacturing Processes > Biological Techniques Areas of Application > Materials and Chemical Industries and Green Manufacturing
Collections:	Nanomanufacturing Research Collection > Nanomanufacturing Nanoscale Science and Engineering Centers > Center for Hierarchical Manufacturing Nanomanufacturing Research Collection
Depositing User:	Moureen Kemei
Date Deposited:	08 Feb 2010 21:25
Last Modified:	19 Sep 2011 21:10
URI:	http://eprints.internano.org/id/eprint/267

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